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Monitoring and robust induction of nephrogenic intermediate mesoderm from human pluripotent stem cells

A method for stimulating the differentiation of human pluripotent stem cells into kidney lineages remains to be developed. Most cells in kidney are derived from an embryonic germ layer known as intermediate mesoderm. Here we show the establishment of an efficient system of homologous recombination in human pluripotent stem cells by means of bacterial artificial chromosome-based vectors and single-nucleotide polymorphism array-based detection. This system allowed us to generate human-induced pluripotent stem cell lines containing green fluorescence protein knocked into OSR1, a specific intermediate mesoderm marker. We have also established a robust induction protocol for intermediate mesoderm, which produces up to 90% OSR1+ cells. These human intermediate mesoderm cells can differentiate into multiple cell types of intermediate mesoderm-derived organs in vitro and in vivo, thereby supplying a useful system to elucidate the mechanisms of intermediate mesoderm development and potentially providing a cell source for regenerative therapies of the kidney.

True to form, when it's not sourced from embryos, AFP and the rest of the mutts in the media won't tell you.

1 posted on 01/23/2013 3:37:35 PM PST by neverdem
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To: neverdem
In 20 years, there will be parts stores....no more donations....and the price of the parts will knock your socks off.

After aging problems of the parts (like Dolly), we'll go back to donations.

2 posted on 01/23/2013 3:47:43 PM PST by Sacajaweau
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To: neverdem

Thank you, Mr. Patriot, for digging for that miniscule item of info. The Brave New World treats scientific facts as if they were spent fuel rods, needing to be buried near the earth’s core, and saving we hoi polloi from contamination.


3 posted on 01/23/2013 3:50:08 PM PST by jobim (.)
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To: neverdem

Bookmark


4 posted on 01/24/2013 2:21:19 AM PST by The SISU kid (I think they taste like Barbie dolls smell.)
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To: neverdem

Bfl


5 posted on 01/24/2013 3:51:10 AM PST by FreedomPoster (Islam delenda est)
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To: neverdem

They need to automate cell passaging; currently human hands and lab air come into close contact with cell cultures, leading to very much contamination and extra work.

When they can use new tech to non-intimately replenish cell culture fluid then a whole host of problems will go away.


6 posted on 01/24/2013 12:01:04 PM PST by gaijin
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To: Pontiac

Bump for later


7 posted on 02/06/2013 2:13:36 AM PST by Pontiac (The welfare state must fail because it is contrary to human nature and diminishes the human spirit.)
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