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Easy amyloid refolding
Highlights in Chemical Biology ^ | 19 February 2010 | Frances Galvin

Posted on 02/20/2010 10:54:33 AM PST by neverdem

Spontaneous refolding of amyloid fibres under mild conditions could provide insight into Alzheimer's disease claim scientists in the US. 

Amyloids are collections of twisted or misfolded proteins and often develop in the brains of people with a number of neurodegenerative diseases, including Alzheimer's and Parkinson's. They have been considered to be the most thermodynamically stable form of protein as very harsh conditions are required to disrupt them. But Igor Lednev and his team at the University of Albany have found amyloid fibres change from one polymorph to another with just mild changes in solution temperature and salinity. 

Lednev hopes this discovery will provide insight into treating neurodegenerative diseases as the biological activity and toxicity of amyloid fibres are determined by their specific polymorph form. 'If we can control the polymorph structure, we might be able to control the toxicity,' he says. 

Amyloid

Protein fibres spontaneously refold under mild environment changes

The discovery was made using Lednev's homebuilt apparatus and a method based on deep ultraviolet resonance Raman spectroscopy (DUVRR), which is very sensitive to protein secondary structure and allows study of the gelatinous, highly light scattering suspensions that insoluble amyloids produce. He says they are perhaps the only group in the world using this technique to study fibre formation. 

Several techniques including DUVRR, atomic force microscopy and hydrogen-deuterium (H-D) exchange experiments, were then used to observe the mature fibres' core structure partially melting and spontaneously refolding when they are transferred from a saline environment at 37 °C to a salt-free solution at 25 °C. The H-D experiments also importantly suggest that the core is not exposed to the solution during refolding, implying it occurs without disintegration, adds Lednev. 

Filip Meersman, who researches protein folding and amyloids at the Catholic University of Leuven, Belgium, finds the work interesting and guesses that this transition 'would likely occur as a function of time as well.' 

Lednev says he believes their combination of DUVRR and H-D exchange could be applied to other fibre polymorphs whose ability to refold might not be easily evident using other techniques. Next the team hope to investigate and understand the phenomenon further. 'Only clear understanding of the mechanism might lead us to a new approach for potentially regulating the biological activity of amyloid fibres,' says Lednev. 

 

 

 

Link to journal article

Amyloid fibrils are alive: spontaneous refolding from one polymorph to another
Dmitry Kurouski, William Lauro and Igor K. Lednev, Chem. Commun., 2010
DOI: 10.1039/b926758a

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TOPICS: Culture/Society; News/Current Events; Technical
KEYWORDS: alzheimers; alzheimersdisease; amyloid; amyloidfibres; duvrrspectroscopy; spectroscopy

1 posted on 02/20/2010 10:54:33 AM PST by neverdem
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To: texas booster

ping


2 posted on 02/20/2010 10:57:42 AM PST by neverdem (Xin loi minh oi)
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To: neverdem
Thank you very much. Now if this line of research can lead to cleaning amyloids from a living brain, then we have a really great story!
3 posted on 02/20/2010 4:05:07 PM PST by texas booster (Join FreeRepublic's Folding@Home team (Team # 36120) Cure Alzheimer's!)
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To: 1066AD; 11Bush; A.Hun; abner; AbsoluteGrace; Advil; aft_lizard; agooga; ahayes; aliquando; ...
Folding@Home ping.

Exciting new research into one of the basic problems in diseased brains.

4 posted on 02/20/2010 4:07:21 PM PST by texas booster (Join FreeRepublic's Folding@Home team (Team # 36120) Cure Alzheimer's!)
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To: quin; Quix; rabscuttle385; Rate_Determining_Step; rdb3; Reactionary; RebelTex; Recovering Hermit; ..
Folding@Home ping.

Exciting new research into one of the basic problems in diseased brains.

5 posted on 02/20/2010 4:08:19 PM PST by texas booster (Join FreeRepublic's Folding@Home team (Team # 36120) Cure Alzheimer's!)
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To: texas booster

Thanks for the ping Texas Booster!


6 posted on 02/20/2010 4:30:46 PM PST by houeto (Remember in November!)
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To: texas booster
Thanks for the ping TB, good article.

Anyone else want to join the Freeper Crew? Click the links:



7 posted on 02/20/2010 5:39:02 PM PST by brityank (The more I learn about the Constitution, the more I realise this Government is UNconstitutional !! )
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To: texas booster
Thanks from Onelife, who, BTW, recently folded his 2,000,000th point...

Please...hold the applause.

8 posted on 02/20/2010 6:44:03 PM PST by Onelifetogive (Flame away...)
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To: texas booster

THANKS.


9 posted on 02/20/2010 6:50:12 PM PST by Quix ( POL Ldrs quotes fm1900 TRAITORS http://www.freerepublic.com/focus/religion/2130557/posts?page=81#81)
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To: texas booster
hey TB, I've literally STOPPED all my folding efforts for the last 6 months.

I was doing over 25k-35k PPD but it was not doing any good for my electric bill :-(

10 posted on 02/20/2010 8:11:03 PM PST by prophetic (0Bama = 1 illegal president = 32 illegal, unconstitutional & unnecessary CZARS to do his job!!)
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To: prophetic
Yeah, GPU folding can pulling in the kilowatts.

The new generation of 32 nm chips is looking like a real winner in points/watt. You can send us your old video cards when/if you upgrade to new toys.

Please feel free to restart when you can with team 36120.

It WILL keep the computer room a few degrees warmer than it would be otherwise!

11 posted on 02/20/2010 9:27:06 PM PST by texas booster (Join FreeRepublic's Folding@Home team (Team # 36120) Cure Alzheimer's!)
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To: texas booster
Exciting new research into one of the basic problems in diseased brains.

What's that?! Mis-folded proteins cause liberalism??

12 posted on 02/20/2010 11:14:05 PM PST by HKMk23 (Jesus loves me, this I know, and He IS coming; sooner than you can possibly imagine!)
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To: texas booster

BTTT


13 posted on 02/21/2010 3:09:45 AM PST by E.G.C.
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To: SunkenCiv; decimon; LucyT; Smokin' Joe
Like, *PING*, dudes.

Cheers!

14 posted on 02/21/2010 9:37:13 AM PST by grey_whiskers (The opinions are solely those of the author and are subject to change without notice.)
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To: texas booster; grey_whiskers

Thanks!


15 posted on 02/22/2010 8:17:07 PM PST by SunkenCiv (Happy New Year! Freedom is Priceless.)
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