In 1929, Alfonso Marcué González, the Basilica's official photographer, took black and white photographs of the Image and after careful examination of the photographic negative, found a clear image of a bearded man reflected in the right eye of the Virgin. He immediately informed the authorities of the Basilica who sworn him to complete silence about the discovery, which he complied.More than 20 years later, on May 29, 1951, Jose Carlos Salinas Chavez, while examining a good photograph of the face, rediscovers the same image of a bearded man reflected in the right eye of the Virgin, in the same place which it could be projected in an alive eye. Since then, many people had the opportunity to examine closely the eyes of the Virgin on the Tilma, including more than 20 physicians, ophthalmologists.
And then there are the celestial images that appear on Mary's cloak. On December 22, 1981, at the Observatory Laplace Mexico City, Father Mario Rojas and Dr. Juan Hernández Illescas, a medical doctor and amateur astronomer, performed an astronomical study of the Image and analyzed the stellar arrangement that appear in the Mantle of Our Lady. They surprisingly discovered that the stars stunningly and accurately map out the various constellations of the Mexican sky. Even more remarkable is the "star map" on the mantle is in the reverse (the cardinal axis rotated 90 degrees counterclockwise): providing a view of the constellations from beyond them, as would be seen looking through them towards the earth. The constellations are consistent with what astronomers believe was in the sky above Mexico City on the day the apparition occurred - in the winter-morning solstice of December 12, 1531, Saturday, at 10:26AM.
And there is more .... Carlos Fernandez del Castillo, a gynecologist, examined the image and has determined that the gynecological measurements of Our Lady's physical dimensions indicated a woman who is pregnant entirely consistent with the stage of pregnancy on December 9th for Jesus birth to occur on Christmas day. A stethoscope was placed below the black band at the waist of Our Lady (a sign that she is pregnant) and heard rhythmic repeating heartbeats at 115 beats per minute, the same as that of a baby in the maternal womb.