+1 One of the best lines from Firefly. My favorite is tied:
Commander Harken: I notice your ship’s called “Serenity”. You were stationed on Hera at the end of the war. Battle of Serenity Valley took place there, if I recall.
Captain Reynolds: [slightly sarcastic] You know, I believe you might be right.
Commander Harken: Independents suffered a pretty crushing defeat there. Some say that after Serenity, the Browncoats were through. That the war ended in that valley.
Captain Reynolds: Hmm.
Commander Harken: Seems odd you’d name your ship after a battle you were on the wrong side of.
Captain Reynolds: May have been the losing side. Still not convinced it was the wrong one.
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Jayne: Don’t much see the benefit in gettin’ involved in stranger’s troubles without a up-front price negotiated.
Shepherd Book: These people need assistance. The benefit wouldn’t necessarily be for you.
Jayne: That’s what I’m sayin’.
Zoë: No one’s gonna force you to go, Jayne. As has been stated, this job is strickly speculative.
Jayne: Good. Don’t know these folks, don’t much care to.
Mal: They’re whores.
Jayne: I’m in.
Simon: I’m trying to put this as delicately as I can... How do I know you won’t kill me in my sleep?
Mal: You don’t know me, son, so let me explain this to you once: If I ever kill you, you’ll be awake. You’ll be facing me, and you’ll be armed.