The Atlantic Avenue Tunnel is officially the world's oldest subway tunnel. This tunnel was built in 1844 beneath a busy street in the City of Brooklyn (Brooklyn did not become part of NYC until a half-century later). The Atlantic Avenue Tunnel is a half-mile long and accommodated two standard gauge railroad tracks.
I’ve been in the Atlantic Avenue one. It runs all the way from the harbor to Flatbush Ave, but most of it was filled in with rubble long ago.