GM antibiotic resistance in China’s rivers

A new study conducted in China finds 6 out of 6 major rivers tested positive for ampicillin antibiotic resistant bacteria [1]. Sequencing of the gene responsible, the blá gene, shows it is a synthetic version derived from a lab and different from the wild type. This suggests to the researchers that synthetic plasmid vectors from genetic engineering applications may be the source of the ampicillin resistance, which is affecting the human population. The blá gene confers resistance to a wide range of therapeutic antibiotics and the widespread environment pollution with blá resistant bacteria is a major public health concern.

The development of antibiotic resistant pathogens, commonly dubbed “superbugs”, are increasingly common due to the overuse of antibiotics in medical and veterinary practices, and the ever-increasing application of genetic engineering to industrial processes including agriculture, biofuel fermentation and environmental remediation on top of laboratory research. Previously, genetic engineering experiments were confined to the laboratory, but with industrial and agricultural applications becoming more common over the last decade, the chances of uncontrolled discharge as well as deliberate release into the environment has widened. One prime example is the planting of genetically modified (GM) crops, many of which carry antibiotic resistant genes.

Genetic engineering uses plasmids – extra-chromosomal DNA molecules that naturally exist in bacteria and other unicellular species – for propagating and manipulating DNA sequences in research and in genetic modification of plants and animals. Plasmids often carry antibiotic resistance marker genes to allow selection with antibiotics for the modified DNA or cells carrying the gene of interest (see [2] (FAQ on Genetic Engineering, ISIS Tutorial). The presence of these antibiotic resistance genes and plasmids in the environment leaves open the possibility of the genes being taken up and transferred into the genetic material of unrelated species of bacteria, some of which may well be serious pathogens.

The transfer of genes directly into the genetic material of cells, bypassing normal reproduction, is referred to as horizontal gene transfer, to distinguish it from the usual vertical gene transfer that occurs in natural reproduction within the same species or in some cases between closely related species.

Scientists including those in ISIS have issued repeated warnings since the 1990s on the dangers of horizontal gene transfer associated with genetic engineering and GM plants and animals that are released into the open environment [3-6] (Gene Technology and Gene Ecology of Infectious Diseases, ISIS scientific publication; Horizontal Gene Transfer – The Hidden Hazards of Genetic Engineering, ISIS/TWN report; GM DNA Does Jump Species, SiS 47; Scientists Discover New Route for GM-gene ‘Escape’, SiS 50), only to be met with denial and dismissal from the proponents and from our regulators.

The new study led by Jun Wen Li at Sechuan University reveals widespread contamination of 6 out of 6 major urban rivers (the Sungari, Haihe, Yellow, Yangtze, Huangpu and Pearl Rivers) with bacteria carrying a synthetic version of the blá gene [1]. The blá gene confers resistance to the most common class of antibiotics called β-lactams, which includes besides ampicillin (a beta-lactam), the penicillin derivatives (penams), cephalosporins (cephems), monobactams, and carbapenems.

The researchers took samples from the rivers, extracted plasmids from bacteria present, and used PCR (polymerase chain reaction) and quantitative real-time PCR to assess the presence of blá DNA. The assay was specific for the blá gene that comprises most recombinant plasmid strains, such as pBR322 and pUC19, both widely used for research and genetic modification. The detection rate varied from 21.9 % (in the Hai He River samples) to 36.4 % (in the Yangtze River samples). The Pearl and Hai He rivers showed the widest range of cephalosporin resistance from the blá gene present in bacterial samples, extending to 3rd- and 4th-generation drugs like cefotaxime and cefoperazone, while the range was narrower (e.g., cefalotin, cephazolin, cefmetazole, and cefoxitin) in samples from the other rivers tested. Analysis confirmed that sequences “neighbouring” the blá sequences “most frequently represented artificial or synthetic constructs, including cloning, expression, shuttle, gene-fusion, and gene trap vectors” derived from recombinant laboratory plasmid vectors, identifying most strongly with pBR322; and confirming the artificial origin of the DNA that does not naturally exist in nature.

Metagenomic technology, which involves transforming environmental genomic DNA into a laboratory recipient strain, is a unique way to study complex genetic samples from ecosystems without purifying the strains. As this study concerned plasmids within environmental microbes, the procedure was modified so that the plasmids were extracted and electro-transformed directly into the laboratory strains. Antibiotics selection was used to identify clones expressing resistant plasmids, which were then isolated and analyzed. A plasmid metagenomic library of 205 environmental plasmid-carrying E. coli HB101 strains was constructed, which showed a positive blá rate of 27.3%. Furthermore, samples from all 6 rivers are also resistant to tetracycline. In addition, some transformants are resistant to other antibiotics such as gentamicin and sulfanilamides. With this technique focusing on plasmids, it is worth noting that plasmid sequences integrated into the bacterial genome were not investigated, and if measured, would likely increase the rate of antibiotic resistant gene contamination further.

The rivers sampled are in highly industrial areas, and the Pearl River in particular was previously reported the most polluted with antibiotics, though the study did not attempt to determine the source of the pollution. What is clear is that once recombinant (GM) plasmids or plasmid sequences are discharged into the environment, the DNA can spread to wild bacteria through the process of horizontal gene transfer. Thus, researchers suggest that horizontal gene transfer of genetically engineered plasmids to microbes in the soil or from lactic acid bacteria to human and animal gut microbes is a likely consequence of such pollution, and may well underlie the rise in antibiotic resistance in animals as well as humans.

But there is another likely major source of GM antibiotic resistance, and that is from GM crops planted in the fields.

The majority of GM crops already released commercially or field trialled in the open environment carry antibiotic resistant genes derived from the synthetic plasmids that were used for genetic modification. China both grows and imports GM foods and trees, many of which harbour the blá gene including: Syngenta’s Bt11 Yieldgard Maize and Bt176 NaturGard Knockout Maize, Monsanto’s Mon21 Roundup Ready Maize and Bayer’s ZM003 Liberty Link Maize. China has also been developing many GM crops, including rice [7]. Bt ‘Shanyou’63, was already the subject of controversy since 2005; the unapproved variety (both in China and other countries) illegally sold and planted in Hubei province, contaminated Chinese rice products exported to Europe and Japan, and has been detected in China and various countries since then. Bt63 was developed in Huazhong Agriculture University in Wuhan, Hubei Province. As recently as July 2009, the European Union called on China to tighten export controls on rice products because shipments might contain traces of the Bt 63 strain, which is not authorized in the European Union [8]. Perhaps it is not a coincidence that the Yangtze River, one of those tested in the study, runs through the Hubei province.

This study is the first to address the potential pollution of our environment with antibiotic resistant genes from genetic engineering experiments. It provides the first comprehensive and direct evidence of horizontal gene transfer from genetic engineering and genetic modification. It can be predicted that similar findings will emerge elsewhere, if the appropriate molecular probes are used with the most sensitive PCR assays, which hitherto has not been done.

China's environmental record is dismal. The eco-nuts here want to classify rain in VA as a pollutant so they can tear down houses, but never see any problem in China. This is because their holiest of hollies (government) has an iron grip on everything.

There are cities in China where westerners can't breathe the air. There are rivers in China that even CARP can't survive in!

Heck, even in the US if a construction site allows a bit of dirt to wash off the site in a heavy rain they get a huge fine. However if a govt controlled sewage plant dumps millions of gallons of raw sewage into the waterways every time it rains that's ok. For info on that just google MMSD (Milwaukee Metro Sewerage District).

We spent a couple months in China (Guangzhou) with my husband who was on a job assignment back in the late 90’s.

I would homeschool in the morning, and then our son and I would explore the city in the afternoon. Some days the smog was so bad, you couldn’t see the buildings across the street.

So many Chinese people have respiratory problems. Everyone was hacking and spitting on the floor seemed to be the “norm” even if you were inside a building or restaurant. I became paranoid about having everyone wash their hands after they put on or took off their shoes because who knows what one had walked through while out on the streets, or in the market (where they were slaughtering animals...cats and dogs no less :) )

We learned that the market where SARS started was the market we visited quite often ( the Qing Ping market.) At the time it was such a culture shock to see the market and the goings on there, it was almost surreal. Supposedly after the SARS outbreak they cleaned up the sanitary situation there.

We were in a 5 star hotel, and the water coming out of the faucet was yellow and smelled awful...the Pearl River was so polluted even in the 90’s.

How can this be? Most of the people there don’t use antibiotics.

Hopefully I've made this clear.

One way is as a side effect of using GM crops. Plasmids that carry antibiotic resistant genes are used to create and are found in GM crops. These genes are transferred to 'wild' bacteria and pathogens. 'horizontally', that is by passing normal reproduction. Proponents of GM crops have denied that this would be possible.

"The majority of GM crops already released commercially or field trialled in the open environment carry antibiotic resistant genes derived from the synthetic plasmids that were used for genetic modification. "

"Genetic engineering uses plasmids – extra-chromosomal DNA molecules that naturally exist in bacteria and other unicellular species – for propagating and manipulating DNA sequences in research and in genetic modification of plants and animals"

More details fro the article:

"Horizontal gene transfer, the hidden hazards of genetic modification

The transfer of genes directly into the genetic material of cells, bypassing normal reproduction, is referred to as horizontal gene transfer, to distinguish it from the usual vertical gene transfer that occurs in natural reproduction within the same species or in some cases between closely related species."

Are these crops in China?

The testing that discovered 'wild' bacteria infected with anti biotic resistant genes unique to GM was done in China, however GM crops are created using similar techniques, and grown world wide.

It was the first such study.

In other words, the potential for this contamination exists anywhere GM crops are created and grown. It just has not been tested for.

Conclusion from the study:

This study is the first to address the potential pollution of our environment with antibiotic resistant genes from genetic engineering experiments. It provides the first comprehensive and direct evidence of horizontal gene transfer from genetic engineering and genetic modification. It can be predicted that similar findings will emerge elsewhere, if the appropriate molecular probes are used with the most sensitive PCR assays, which hitherto has not been done.

Are these crops in China?

I forgot to add that proponents of GM have used scientists to deny the possibility of horizontal gene transference just like they denied the possibility of pollen from GM crops infecting nearby non GM fields.

The way the 'science' works is 'scientists' claim 'this' has never been proven to happen, therefore 'this' can't happen. In reality they either never tested for 'this' or tested for 'this' for such a short period of time that the test was not valid.

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