The Presbyterians were the reason the articulation of a right to freedom of religion was necessary for the Constitution to be ratified. Less than 100 years before the Declaration of Independence it was illegal to be Catholic in many of the colonies and as late as 1654 Massachusetts actually executed 10 people for being Catholic. Although only 1.6% of colonists were Catholic estimates were that over 5% of all colonial troops were Catholic. Ironically, it was the contributions of heavily Catholic Pennsylvania militia, the intervention of Catholic France and Spain and the assistance of de Kalb and Pulaski that secured our independence.
Here in NC, the independence movement was a Baptist affair. A large share of the Regulators were Baptists, and the back-country in the state was heavily Baptist (the Presbyterians were mostly represented among the Highland Scots (loyalists, for the most part) in the Cape Fear region as well as among Scots-Irish in the Piedmont (from among whom, however, many of the Baptist converts were made).
John Leland and other Virginia Baptists were the ones who prevailed upon Jefferson and especially Madison to include the language of religious freedom (rather than mere toleration) in the 1st amendment.