There was no canon until after 300 A.D.
And there was no officially defined canon until the Council of Trent in the 16th century. Up until that time there wasn't a reason to do so since virtually all Western Christians used the 73 book canon in use by Catholics today. It wasn't until Martin Luther started removing books from the Bible (on his own authority) to fit his own novel theology that the Church finally was forced to define the canon. Before that it was virtually unquestioned.