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False-Negative Results of Plasma of Patients with Severe Viral Hemorrhagic Fever (Ebola)
Journal of Clinical Microbiology ^ | November 2002 | Christian Drosten,* Marcus Panning, Stephan Guenther, and Herbert Schmitz

Posted on 08/03/2014 10:42:08 PM PDT by mojito

Viral hemorrhagic fevers (VHF) are acute infections with high case fatality rates, associated with the risk of nosocomial transmission (3). A rapid confirmation of the clinical diagnosis is therefore required by methods such as antigen capture enzyme immunoassay and serologic detection of immunoglobulin M. With the development of PCR technology, it has become possible to rapidly test for viruses that cause VHF (4, 5, 10). We have recently confirmed a case of acute yellow fever with fulminating hepatic failure by reverse transcription-PCR (RT-PCR) (2). In spite of the very high viral RNA concentration in the plasma sample, it initially tested negative. A confirmation by PCR would have been missed if we had not tested a duplicate sample...

(Excerpt) Read more at ncbi.nlm.nih.gov ...


TOPICS: Health/Medicine; Science
KEYWORDS: ebola; falsenegative
Translation: false negatives are common when testing for Ebola and Ebola-like viruses.

Title shortened for space reasons. Full title: False-Negative Results of PCR Assay with Plasma of Patients with Severe Viral Hemorrhagic Fever.

1 posted on 08/03/2014 10:42:08 PM PDT by mojito
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To: mojito
And still a problem:

Liberia: Catholic Hospital Boss Tested Positive of Ebola - Report

“According to cogent information gathered by this paper, Rev. Brother Patrick Nshamdze, was tested positive after he supposedly came in contact with a patient who died from the virus.

Our information divulged that on the 17th of this month, his specimen was taken and the result, which came on the next day (July 18), proved negative.

Not being satisfied, the report further divulged, the ailing Catholic hospital director decided to seek further treatment abroad, but his trip was subjected to Ebola test.

It was based on this that he did another test on Tuesday, July 29, 2014, which proved positive, contrary to the first test he underwent.

Rev. Bro. Nshamdze is currently being quarantined at the Catholic Hospital undergoing treatment.

The hospital authorities are contemplating on shutting down the hospital for two weeks for disinfection and chlorination of the entire building.”

http://allafrica.com/stories/201407310957.html

2 posted on 08/03/2014 10:47:11 PM PDT by mojito (Zero, our Nero.)
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To: mojito

That could explain how it is transmitting faster then it should. Too many false negatives.


3 posted on 08/03/2014 10:56:09 PM PDT by justa-hairyape (The user name is sarcastic. Although at times it may not appear that way.)
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To: mojito
http://www.promedmail.org/direct.php?id=20121116.1412991

Date: Fri 16 Nov 2012 Source: BBC World Service, Science and Environment [edited] http://www.bbc.co.uk/news/science-environment-20341423

Canadian scientists have shown that the deadliest form of Ebolavirus could be transmitted by air between species.

In experiments, they demonstrated that the virus was transmitted from pigs to monkeys without any direct contact between them. The researchers say they believe that limited airborne transmission might be contributing to the spread of the disease in some parts of Africa. They are concerned that pigs might be a natural host for the lethal infection.

---

Meanwhile passengers from suspect African cities are arriving on international flights, with minimal screening only for obvious symptoms, for a disease that is extremely contagious and that has an incubation period of up to 21 days.

It's a matter of time before it goes pandemic, if it hasn't already.

4 posted on 08/03/2014 11:07:39 PM PDT by zipper (In Their Heart Of Hearts, Every Democrat Is A Communist.)
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To: mojito

There will need to be a reliable screening method to determine who has contracted the Ebola virus. People are contagious when they don’t yet show symptoms. This is the time to start the screening process in the vector countries so those who are infected can be quarantined.


5 posted on 08/03/2014 11:34:46 PM PDT by jonrick46 (The opium of Communists: other people's money.)
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To: mojito; Black Agnes
This is a good post. Kent Brantly originally tested neg when he was already having symptoms, then a day or two later he tested positive.

Where was it that the woman fleeing Sierra Leone collapsed and then her test was negative?

6 posted on 08/03/2014 11:34:55 PM PDT by MarMema (Run Ted Run)
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To: zipper
Supposedly, the woman on the flight to London tested negative.

But even if the woman is truly negative, it's only a matter of time. There are now confirmed cases in Lagos, Nigeria and Morocco.

A mutated form of Ebola that could be transmitted by air would explain a lot. Like the fact that many of the healthcare workers in Liberia and other hot zones were contracting the disease even though they were wearing hazmat suits.

7 posted on 08/03/2014 11:36:33 PM PDT by mojito (Zero, our Nero.)
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To: MarMema
England.
8 posted on 08/03/2014 11:37:50 PM PDT by Chgogal (Obama "hung the SEALs out to dry, basically exposed them like a set of dog balls..." CMH)
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To: MarMema
At London's Gatwick Airport.
9 posted on 08/03/2014 11:38:10 PM PDT by mojito (Zero, our Nero.)
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To: MarMema
FYI

http://www.freerepublic.com/focus/news/3188557/posts?page=122#122

10 posted on 08/03/2014 11:39:08 PM PDT by Chgogal (Obama "hung the SEALs out to dry, basically exposed them like a set of dog balls..." CMH)
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To: mojito
You don't need airborne because droplet precautions are three feet and it lives in dried material for days.

That's virulent enough on its own merit.

11 posted on 08/03/2014 11:49:00 PM PDT by MarMema (Run Ted Run)
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To: mojito

Yep.

This has been my concern.

Time will soon tell if any of these false-negatives turn into positives.


12 posted on 08/04/2014 12:42:14 AM PDT by Shelayne
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To: mojito

Seven suspected cases in the Philippines as well from workers returning from Sierra Leone.

http://english.cntv.cn/2014/08/02/VIDE1406955241574888.shtml


13 posted on 08/04/2014 12:58:16 AM PDT by Shelayne
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To: Smokin' Joe; null and void

False negatives are common when testing for Ebola and Ebola-like viruses.


14 posted on 08/04/2014 1:13:36 AM PDT by Covenantor ("Men are ruled...by liars who refuse them news, and by fools who cannot govern." Chesterton)
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To: 2ndreconmarine; Fitzcarraldo; Covenantor; Mother Abigail; EBH; Dog Gone; ...

Ping...


15 posted on 08/04/2014 3:17:59 AM PDT by Smokin' Joe (How often God must weep at humans' folly. Stand fast. God knows what He is doing.)
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To: mojito

Don’t hazmat suits have particle masks that would shield from an airborne virus?


16 posted on 08/04/2014 5:35:07 AM PDT by Jedidah
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To: mojito; neverdem; ProtectOurFreedom; Mother Abigail; EBH; vetvetdoug; Smokin' Joe; Global2010; ...
Well! You're just one big frickin' ray of sunshine, aren't you???

Bring Out Your Dead

Post to me or FReep mail to be on/off the Bring Out Your Dead ping list.

The purpose of the “Bring Out Your Dead” ping list (formerly the “Ebola” ping list) is very early warning of emerging pandemics, as such it has a high false positive rate.

So far the false positive rate is 100%.

At some point we may well have a high mortality pandemic, and likely as not the “Bring Out Your Dead” threads will miss the beginning entirely.

*sigh* Such is life, and death...

17 posted on 08/04/2014 6:30:07 AM PDT by null and void (If Bill Clinton was the first black president, why isn't Barack Obama the first woman president?)
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To: jonrick46; ansel12
People are contagious when they don’t yet show symptoms. This is the time to start the screening process in the vector countries so those who are infected can be quarantined.

Unless they are Americans, then we should send a private jet to bring them here, right ansel???

18 posted on 08/04/2014 6:32:51 AM PDT by null and void (If Bill Clinton was the first black president, why isn't Barack Obama the first woman president?)
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To: mojito

Well, it could be that lady who died on the flight to Britain may well have had ebola. Or it could be she needed second test.


19 posted on 08/04/2014 6:36:10 AM PDT by dforest
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To: null and void; moder_ator

Seriously, lay off the stalking and pinging me to threads that I’m not even on.

If you want to continue a thread, then go back to the one that you disagreed with me on, don’t start dragging it throughout FR.


20 posted on 08/04/2014 7:38:54 AM PDT by ansel12 (LEGAL immigrants, 30 million 1980-2012, continues to remake the nation's electorate for democrats)
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To: ansel12

You don’t like daylight?


21 posted on 08/04/2014 7:42:18 AM PDT by null and void (If Bill Clinton was the first black president, why isn't Barack Obama the first woman president?)
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To: null and void

It’s called stalking, don’t carry a disagreement from one thread throughout FR and installing it on fresh threads, if you have a point you want to make on a thread, then return to that original thread and make it.


22 posted on 08/04/2014 7:47:59 AM PDT by ansel12 (LEGAL immigrants, 30 million 1980-2012, continues to remake the nation's electorate for democrats)
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To: Jedidah
I don't know enough about the respirators on hazmat suits and how they function to answer your question.

I would guess that there may be several kinds.

Maybe some other FReepers can answer.

23 posted on 08/04/2014 8:57:26 AM PDT by mojito (Zero, our Nero.)
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To: mojito

good tag line

unfortunately


24 posted on 08/04/2014 11:45:31 AM PDT by SisterK (the great tribulation begins)
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To: null and void

People who are contagious with Ebola can slip into the US without anyone knowing the are contagious. There has to be a reliable method of detecting them so they can be quarantined before they infect others.


25 posted on 08/04/2014 12:21:19 PM PDT by jonrick46 (The opium of Communists: other people's money.)
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To: jonrick46

Quarantined??? Don’t be silly. There’s a FReeper on this very thread who advocates sending a private jet out to bring Ebola patients here.


26 posted on 08/05/2014 7:19:11 AM PDT by null and void (If Bill Clinton was the first black president, why isn't Barack Obama the first woman president?)
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To: piytar
Here is another for your list. In the lab we call this a prozone effect.

Or you can just call it testing inaccuracies...which add to the mess we are already in now.

27 posted on 10/02/2014 2:47:56 AM PDT by MarMema (Run Ted Run)
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