 The monks have vowed to continue their action
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Lorries with loudspeakers have been driving through Burma's main city of Rangoon warning residents to stop anti-government protests.
The broadcasts threatened that "action will be taken against those who violate this order".
But hundreds of monks and civilians defied the threats and began fresh protests at the Shwedagon pagoda.
On Monday, there were protests in at least 25 towns, with tens of thousands of people marching in Rangoon.
Several military trucks are now parked near Shwedagon pagoda, which has been the focus of the protests.
Eyewitnesses said several hundred monks gathered at Shwedagon with the apparent intention of marching again.
The BBC's Jonathan Head in Bangkok says monks - who have been spearheading the protest campaign - have been handing out pictures of Burmese independence hero Aung San, the deceased father of detained opposition leader Aung San Suu Kyi.
They are also carrying flags, including some bearing the image of a fighting peacock used by students during the 1988 pro-democracy uprising, witnesses told Reuters news agency.
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PROTESTS MOUNT
15 Aug: Junta doubles fuel prices, sparking protests
5 Sept: Troops injure several monks at a protest in Pakokku
17 Sept: The junta's failure to apologise for the injuries draws fresh protests by monks
18-21 Sept: Daily marches by monks in Burmese cities gradually gather in size
22 Sept: 1,000 monks march to the home of Aung San Suu Kyi in Rangoon
23 Sept: Up to 20,000 march in Rangoon
24 Sept: New Rangoon march draws at least 50,000 and 24 other towns join in
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The junta, which violently repressed the 1988 protests killing some 3,000 people, finally broke its silence over the mounting protests, saying late on Monday that it was ready to "take action" against the monks.
It repeated its warning in state media on Tuesday, ordering monks not to get involved in politics and accusing them of allowing themselves to be manipulated by the foreign media.
US President George W Bush is to announce further sanctions against Burma's ruling military junta in response to the protests, the White House has said.
Mr Bush is expected to announce the new restrictions during his speech to the United Nations General Assembly in New York. Washington is hoping it will encourage other nations to act and embolden the protesters on Burma's streets, says the BBC's Jonathan Beale in Washington.
The authorities are likely to be under huge pressure from their close neighbour China to avoid bloodshed and instability, the BBC's Asia correspondent, Andrew Harding notes.
The European Union has also urged the junta to show the "utmost restraint" in dealing with the protests and to take the opportunity to "launch a process of real political reform".
The exiled Tibetan spiritual leader, the Dalai Lama, has given his backing to the monks' call for freedom and democracy.
Detained leader
A group of more than 1,000 of the maroon-robed monks and 400 sympathisers went to Aung San Suu Kyi's street at the end of Monday's march, the Associated Press reported.
They chanted a prayer for peace in the face of the riot police blocking access to her home, where she is under house arrest, before dispersing peacefully.
The organisation that has emerged to lead the protests, the Alliance of All Burmese Buddhist Monks, has vowed to continue marches until it has "wiped the military dictatorship from the land".
The protests were triggered by the government's decision to double the price of fuel last month, hitting people hard in the impoverished nation.
[Bonus picture] 
