Posted on 03/13/2020 9:29:51 AM PDT by Hostage
Abstract
The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome (MERS)-CoV underscores the threat of cross-species transmission events leading to outbreaks in humans. Here we examine the disease potential of a SARS-like virus, SHC014-CoV, which is currently circulating in Chinese horseshoe bat populations1. Using the SARS-CoV reverse genetics system2, we generated and characterized a chimeric virus expressing the spike of bat coronavirus SHC014 in a mouse-adapted SARS-CoV backbone. The results indicate that group 2b viruses encoding the SHC014 spike in a wild-type backbone can efficiently use multiple orthologs of the SARS receptor human angiotensin converting enzyme II (ACE2), replicate efficiently in primary human airway cells and achieve in vitro titers equivalent to epidemic strains of SARS-CoV. Additionally, in vivo experiments demonstrate replication of the chimeric virus in mouse lung with notable pathogenesis. Evaluation of available SARS-based immune-therapeutic and prophylactic modalities revealed poor efficacy; both monoclonal antibody and vaccine approaches failed to neutralize and protect from infection with CoVs using the novel spike protein. On the basis of these findings, we synthetically re-derived an infectious full-length SHC014 recombinant virus and demonstrate robust viral replication both in vitro and in vivo. Our work suggests a potential risk of SARS-CoV re-emergence from viruses currently circulating in bat populations.
(Excerpt) Read more at nature.com ...
Note the following under methods and connect the dots:
"All virus cultivation was performed in a biosafety level (BSL) 3 laboratory with redundant fans in the biosafety cabinets, as described previously by our group."
==> Cabinets, Biosafety Cabinets.
The weaponized virus was not stored in secure facilities monitored by military security units. It was stored in labs in cabinets.
Kinda like storing a nuclear bomb in a university physics lab.
Now let's get smart and listen to Dr. Judy Mikovits:
https://www.youtube.com/watch?v=ALHm8WTGOkA&feature=youtu.be
You will learn a little about microbiology but importantly that all these retroviruses have a common intersection.
Oh, and the DOW is soaring today.
Democrats are sad.
But it might bite again on Monday.
Democrats will be jubilant.
Then on Tuesday it might roar back.
Democrats will be sad.
Rinse and repeat.
English please
Providing protection but not security.
Isn’t that cute?
Biosafety Cabinet. Not a standard storage cabinet.
It’s a funny-sounding term, but it’s a secure safety enclosure.
Pretty sure Corona isn’t a “retro virus”. It’s single strand RNA.
It’s in English.
If you can’t read it, sue your science teacher or your parents or your Rabbi or Santa Claus or join the Bernies and sue all capitalists for all time.
It’s not secure, safe yes, secure nyet.
Oops! I let my Russian slip.
“All mouse studies were performed at the University of North Carolina “
It’s Covid 19, it’s a retrovirus. Corona is a component only.
I wonder what Greta Thunberg has to say about Wuhan Flu?
Interesting. Thanks.
Bookmark
Technicalities aside, you make a good point. The world was asleep while potential doomsday viruses were being engineered.
Fruit bats didn’t just fly hundreds of miles from the jungle into a highly industrialized zone like Wuhan.
When this is over, there will be a massive day of reckoning for the globalists who allowed China to develop this stuff.
Cows don’t go there either, but you can find beef.
She must be thanking God (oops, I mean Thor, or more likely Satan).
Most of the work seems to have been done in the US, led by the University of Chapel Hill, but some was done at Wuhan:
“Pseudotyping experiments were similar to those using an HIV-based pseudovirus, prepared as previously described10, and examined on HeLa cells (Wuhan Institute of Virology) that expressed ACE2 orthologs.”
The people involved in China (under Author - Affiliations)
“Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
Xing-Yi Ge & Zhengli-Li Shi”
And the work the Chinese scientists did (listed as X.-Y.G. and Z.-L.S):
“X.-Y.G. performed pseudotyping experiments”
“Z.-L.S. provided SHC014 spike sequences and plasmids”
What is “pseudotyping”?:
“Pseudotyping is the process of producing viruses or viral vectors in combination with foreign viral envelope proteins. The result is a pseudotyped virus particle. With this method, the foreign viral envelope proteins can be used to alter host tropism or an increased/decreased stability of the virus particles.”
“Pseudotyping experiments were similar to those using an HIV-based pseudovirus, prepared as previously described10, and examined on HeLa cells (Wuhan Institute of Virology) that expressed ACE2 orthologs.”
I do believe there is a record of that lab in that year (perhaps after the fed regulation limited their continued work on it?) selling it to the ChiComs with Obama admin approval.
Please dig and find.
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