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Coronavirus Live Thread. Post 2
2/24/2020

Posted on 02/24/2020 2:14:53 PM PST by Vermont Lt

Just think. Ten months till Christmas.

This is the continuation of the Thread.


TOPICS: Chit/Chat; Health/Medicine
KEYWORDS: apocalypse; billionsinfected; chinavirus; coronabeer; coronavirus; covid19; cvlivethread; everyonedies; fart; flu; globaldoom; hysteria; livethread; millionsdead; ohnoes; rumors; sarscov2; speculation
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To: blueplum

John Kerry been there lately?

a boy can dream can’t he?


501 posted on 02/25/2020 5:00:18 PM PST by Pollard (If you don't understand what I typed, you haven't read the classics.)
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To: ETCM

It can probably be tested by taking a bodily fluid sample and looking at it through an electron microscope. Plenty of color pics of the little bugger on the web. Not everyone has an electron scope though and it’s subject to human error/interpretation. Hence they need test kits for dummies. Why the first batch were defective? Who knows. Made in China? Procured by some RESIST person in fedgov? Incompetence on someone’s part?

Meanwhile Trump shut off travel of people coming here from China early this month and since 77,000+ cases out of 80,000 cases are in China, that seems to have given us time for the test kit glitch.

But this ain’t the flu. All we can do is keep track and prepare ourselves. So glad I moved from Ctrl FL to rural Ozarks.


502 posted on 02/25/2020 5:18:42 PM PST by Pollard (If you don't understand what I typed, you haven't read the classics.)
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To: LilFarmer

Another reply was, “But nothing for the shit-covered streets and increasing homeless problem?”

I replied to him with;

“Nanc is probably hoping the virus will kill most of them off. Hell, she’ll probably have DiFi’s driver deliver some infected people personally”


503 posted on 02/25/2020 5:25:45 PM PST by Pollard (If you don't understand what I typed, you haven't read the classics.)
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To: LilFarmer

People can walk around carrying and spreading this thing while testing negative for 7-28 days depending on the source.

What I want to know is, are these other countries using test kits or looking at bodily fluid samples through microscopes?

Our fedgov is all about contracts so are they just waiting to procure actual working test kits for dummies?


504 posted on 02/25/2020 5:37:37 PM PST by Pollard (If you don't understand what I typed, you haven't read the classics.)
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To: Pollard

^


505 posted on 02/25/2020 5:38:14 PM PST by abigkahuna (How can you be at two places at once when you are nowhere at all?)
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To: Pollard
A microscope is fine for morphology. When bacteria are the topic, a Gram stain and view under an optical microscope tells you Gram positive (purple) or Gram negative (pink from the saffranin counter-stain). You see rods, spheres or spirals. Singular, pairs, quads in spheres. Length and width. Lots of observables. Optical scopes do nothing for viruses. You need an electron microscope. Stains with lead or gold for transmission scopes. Sputter coated with metals for the scanning EM.

After morphology, there is physiology. What media does it like, what metabolites does it produce? What temperatures does it like? Going deeper, there is the antigen signature. Use of labeled antibodies that latch onto a known antigen target can confirm a serotype.

The level of examination required for this corona virus requires breaking up the encapsulation and getting to the RNA inside. You have to amplify the tiny amount present with a polymerase chain reaction to make a huge number of identical copies. At that point you have the means to start sequencing the RNA an comparing the sequences to databases of known viruses or organisms. All of that finery was invented well after I graduated from UCSD in 1976. We did sequencing the hard way. Machines do that today.

It's not a simple process. Perhaps that's why CDC has ONE bench available and takes 6 hours to complete one test.

506 posted on 02/25/2020 6:45:36 PM PST by Myrddin
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To: Myrddin

Ah ok. Beyond me. Electron microscope, I’ve heard of. So how are all these other countries testing while we can’t?


507 posted on 02/25/2020 7:19:59 PM PST by Pollard (If you don't understand what I typed, you haven't read the classics.)
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To: Pollard
I will hazard a guess given my out of date experience. The news articles reference doing a "nucleic acid" test. The initial steps to break the capsid and get the RNA out, purify and amplify are the same preliminary steps. Install of a full blown sequencing, you can create a detection medium that binds only to a specific known target. Add an indicator that changes color when the detection medium successfully binds to the expected target.
508 posted on 02/25/2020 7:46:51 PM PST by Myrddin
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To: LilFarmer

It’s kinda like when I’ve read comments from Europeans this winter that they’ve had influenza A or influenza B I gathered that our medical system has more of “relaxed” attitude about viruses. I’ve never had a swab for a virus even when I was very ill in bed with what I assumed was flu but called the doctor on the 8th day when fever still hadn’t broke and he called in an antibiotic. It worked, thank the Lord, but I never did find out which “strain” I had or even a confirmation it was flu.


509 posted on 02/25/2020 11:26:10 PM PST by kelly4c
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To: ETCM

last i saw was theyd be ready mid march have you heard otherwise..i hope so


510 posted on 02/25/2020 11:36:07 PM PST by kelly4c
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To: Myrddin

great diagram, thanks!


511 posted on 02/26/2020 12:48:19 PM PST by blueplum ( ("...this moment is your moment: it belongs to you... " President Donald J. Trump, Jan 20, 2017))
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