Posted on 12/29/2009 1:11:08 PM PST by decimon
Milwaukee, WI – Researchers from the Medical College of Wisconsin, the Children's Research Institute, and the Children's Hospital of Wisconsin have developed a rapid, automated system to differentiate strains of influenza. The related report by Beck et al, "Development of a rapid automated influenza A, influenza B, and RSV A/B multiplex real-time RT-PCR assay and its use during the 2009 H1N1 swine-origin influenza virus (S-OIV) epidemic in Milwaukee, Wisconsin," appears in the January 2010 issue of the Journal of Molecular Diagnostics.
In pandemic infection, such as the present H1N1 influenza outbreak, rapid automated tests are needed in order to make quick and effective public health decisions. Real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR) is a sensitive and specific method for identifying flu substrains; however, technician and assay time are significantly longer than less accurate rapid influenza diagnostic tests.
A group led by Dr. Kelly J. Henrickson of the Medical College of Wisconsin have developed rapid semi- and fully-automated multiplex real-time RT-PCR assays to detect influenza A, influenza B, and respiratory syncytial virus (RSV). These assays can successfully detect human H1N1, H3N2, and swine-origin H1N1 viruses as well as distinguish these from influenza B and RSV infections. These assays could test large numbers of samples over a very short time, allowing for a significant decrease in both technician and assay time.
Beck et al suggest that "this outbreak demonstrates the importance of having rapid, reliable, sensitive, and specific assays that allow clinicians and public health officials to react quickly and effectively during viral outbreaks."
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These studies were funded by the Midwest Respiratory Virus Program Clinical Laboratory.
Beck, ET, Jurgens LA, Kehl SC, Bose ME, Patitucci T, LaGue E, Darga, P, Wilkinson K, Witt LM, Fan J, He J, Kumar S, Henrickson KJ: Development of a rapid automated influenza A, influenza B, and RSV A/B multiplex real-time RT-PCR assay and its use during the 2009 H1N1 swine-origin influenza virus (S-OIV) epidemic in Milwaukee, Wisconsin. J Mol Diagn 2010, 12:74-81
For press copies of the articles, please contact Dr. Angela Colmone at 301-634-7953 or acolmone@asip.org.
The Journal of Molecular Diagnostics, the official publication of the Association for Molecular Pathology, co-published by the American Society for Investigative Pathology, seeks to publish high quality original papers on scientific advances in the translation and validation of molecular discoveries in medicine into the clinical diagnostic setting, and the description and application of technological advances in the field of molecular diagnostic medicine. The editors welcome for review articles that contain: novel discoveries or clinicopathologic correlations including studies in oncology, infectious diseases, inherited diseases, predisposition to disease, or the description or polymorphisms linked to disease states or normal variations; the application of diagnostic methodologies in clinical trials; or the development of new or improved molecular methods for diagnosis or monitoring of disease or disease predisposition.
Real-time ping.
thanks, bfl
During the S-OIV outbreak in Milwaukee, WI, the fully automated assay was used to test 1232 samples in 2 weeks.
Despite the press release giving the title in quotation marks, i.e. in the manner in which I search for any statement on the Internet, Yahoo found nothing, and Google only found this and other press releases. I had to go to the Journal of Molecular Diagnostics and go down the table of contents to find this abstract.
Doesn’t say what the tests cost but this sounds good. This year it seemed that every day there was a new and contradictory report of how many seasonal and how many H1N1 flu cases there were.
Also, keep up with other H1N1 update stories on this thread: H1N1 flu victim collapsed on way to hospital [Latest H1N1 updates downthread] thanks to DvdMom and others.
Thanks for the ping!
Jeffrey's mom: Jeffrey, I really don't like your friends.
Jeffrey: Try the broccoli
"Some of you may find this to be a big surprise, but a Harvard study shows that the great swine flu pandemic was oversold. Shocking I know, as one doctor points out, “The H1N1 pandemic was a pandemic that never materialized.” Then I guess you could call the swine flu pandemic oversold, wouldn’t you think?"
I ping folks to all the articles, and let them make up their own minds.
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