Free Republic
Browse · Search
Topics · Post Article

Skip to comments.

New Route For Heredity Bypasses DNA
ScienceDaily ^ | (Jan. 4, 2008) | ScienceDaily(Princeton University)

Posted on 01/04/2008 8:35:22 PM PST by Maelstorm

ScienceDaily (Jan. 4, 2008) — A group of scientists in Princeton's Department of Ecology and Evolutionary Biology has uncovered a new biological mechanism that could provide a clearer window into a cell's inner workings.

What's more, this mechanism could represent an "epigenetic" pathway -- a route that bypasses an organism's normal DNA genetic program -- for so-called Lamarckian evolution, enabling an organism to pass on to its offspring characteristics acquired during its lifetime to improve their chances for survival. Lamarckian evolution is the notion, for example, that the giraffe's long neck evolved by its continually stretching higher and higher in order to munch on the more plentiful top tree leaves and gain a better shot at surviving.

The research also could have implications as a new method for controlling cellular processes, such as the splicing order of DNA segments, and increasing the understanding of natural cellular regulatory processes, such as which segments of DNA are retained versus lost during development. The team's findings will be published Jan. 10 in the journal Nature.

Princeton biologists Laura Landweber, Mariusz Nowacki and Vikram Vijayan, together with other members of the lab, wanted to decipher how the cell accomplished this feat, which required reorganizing its genome without resorting to its original genetic program. They chose the singled-celled ciliate Oxytricha trifallax as their testbed.

Ciliates are pond-dwelling protozoa that are ideal model systems for studying epigenetic phenomena. While typical human cells each have one nucleus, serving as the control center for the cell, these ciliate cells have two. One, the somatic nucleus, contains the DNA needed to carry out all the non-reproductive functions of the cell, such as metabolism. The second, the germline nucleus, like humans' sperm and egg, is home to the DNA needed for sexual reproduction.

When two of these ciliate cells mate, the somatic nucleus gets destroyed, and must somehow be reconstituted in their offspring in order for them to survive. The germline nucleus contains abundant DNA, yet 95 percent of it is thrown away during regeneration of a new somatic nucleus, in a process that compresses a pretty big genome (one-third the size of the human genome) into a tiny fraction of the space. This leaves only 5 percent of the organism's DNA free for encoding functions. Yet this small hodgepodge of remaining DNA always gets correctly chosen and then descrambled by the cell to form a new, working genome in a process (described as "genome acrobatics") that is still not well understood, but extremely deliberate and precise.

Landweber and her colleagues have postulated that this programmed rearrangement of DNA fragments is guided by an existing "cache" of information in the form of a DNA or RNA template derived from the parent's nucleus. In the computer realm, a cache is a temporary storage site for frequently used information to enable quick and easy access, rather than having to re-fetch or re-create the original information from scratch every time it's needed.

"The notion of an RNA cache has been around for a while, as the idea of solving a jigsaw puzzle by peeking at the cover of the box is always tempting," said Landweber, associate professor of ecology and evolutionary biology. "These cells have a genomic puzzle to solve that involves gathering little pieces of DNA and putting them back together in a specified order. The original idea of an RNA cache emerged in a study of plants, rather than protozoan cells, though, but the situation in plants turned out to be incorrect."

Through a series of experiments, the group tested out their hypothesis that DNA or RNA molecules were providing the missing instruction booklet needed during development, and also tried to determine if the putative template was made of RNA or DNA. DNA is the genetic material of most organisms, however RNA is now known to play a diversity of important roles as well. RNA is DNA's chemical cousin, and has a primary role in interpreting the genetic code during the construction of proteins.

First, the researchers attempted to determine if the RNA cache idea was valid by directing specific RNA-destroying chemicals, known as RNAi, to the cell before fertilization. This gave encouraging results, disrupting the process of development, and even halting DNA rearrangement in some cases.

In a second experiment, Nowacki and Yi Zhou, both postdoctoral fellows, discovered that RNA templates did indeed exist early on in the cellular developmental process, and were just long-lived enough to lay out a pattern for reconstructing their main nucleus. This was soon followed by a third experiment that "… required real chutzpah," Landweber said, "because it meant reprogramming the cell to shuffle its own genetic material."

Nowacki, Zhou and Vijayan, a 2007 Princeton graduate in electrical engineering, constructed both artificial RNA and DNA templates that encoded a novel, pre-determined pattern; that is, that would take a DNA molecule of the ciliate's consisting of, for example, pieces 1-2-3-4-5 and transpose two of the segments, to produce the fragment 1-2-3-5-4. Injecting their synthetic templates into the developing cell produced the anticipated results, showing that a specified RNA template could provide a new set of rules for unscrambling the nuclear fragments in such a way as to reconstitute a working nucleus.

"This wonderful discovery showed for the first time that RNA can provide sequence information that guides accurate recombination of DNA, leading to reconstruction of genes and a genome that are necessary for the organism," said Meng-Chao Yao, director of the Institute of Molecular Biology at Taiwan's Academia Sinica. "It reveals that genetic information can be passed on to following generations via RNA, in addition to DNA."

The research team believes that if this mechanism extends to mammalian cells, then it could suggest novel ways for manipulating genes, besides those already known through the standard methods of genetic engineering. This could lead to possible applications for creating new gene combinations or restoring aberrant cells to their original, healthy state.

Support for the team's research was provided by the National Science Foundation, the National Institutes of Health and the School of Engineering and Applied Science senior thesis research fund.

TOPICS: Culture/Society; News/Current Events
KEYWORDS: dna; epigenetic; germline; godsgravesglyphs; heredity; rnacache
Navigation: use the links below to view more comments.
first previous 1-2021-34 last
To: editor-surveyor
It appears that reality may have slammed the evos upside their little heads.

So "Lamarckian evolution" (like the Soviets once tried to support) confirms creationism?

21 posted on 01/05/2008 12:04:18 PM PST by Captain Pike
[ Post Reply | Private Reply | To 20 | View Replies]

To: Captain Pike
So "Lamarckian evolution" (like the Soviets once tried to support) confirms creationism?


Lamarck proved that cleaving to the evolving dialectic of evolution through acquired charteristics created more & more research grants from Stalin, thus keepiing Lamarck from acquiring the undesirable characteristic of a bullet hole in his cranium.

22 posted on 01/05/2008 12:57:43 PM PST by ApplegateRanch (If God didn't want a Liberal/RINO hanging from every tree, He wouldn't have created so much rope!)
[ Post Reply | Private Reply | To 21 | View Replies]

To: SunkenCiv

“Junk” RNA regulates important cellular processes
Medical Research News
Published: Tuesday, 9-May-2006

‘”We may even dare to maintain that proteins are presumably responsible only for the basic structure and function of the cell, while RNA is used for its regulation,” says Mayer. Hence, RNA molecules are a central part of cellular regulation processes and more important than previously thought.’

23 posted on 01/05/2008 2:53:16 PM PST by Fred Nerks (FAIR DINKUM!)
[ Post Reply | Private Reply | To 16 | View Replies]

To: Maelstorm

I don’t think you should put too much stock in this.

The conclusions seems far in excess of the discovery (and it is not a new discovery, but an old one that has been dredged up to fit a conclusion for which there is no application to complex, biological life).

24 posted on 01/05/2008 3:01:35 PM PST by coconutt2000 (NO MORE PEACE FOR OIL!!! DOWN WITH TYRANTS, TERRORISTS, AND TIMIDCRATS!!!! (3-T's For World Peace))
[ Post Reply | Private Reply | To 1 | View Replies]

To: coconutt2000

I think there is plenty of stock to put in it. You are right it is not entirely “new”.

25 posted on 01/05/2008 3:26:10 PM PST by Maelstorm (A candidate that does not believe America is the best country on earth need not apply.)
[ Post Reply | Private Reply | To 24 | View Replies]

To: Captain Pike; ApplegateRanch
"So "Lamarckian evolution" (like the Soviets once tried to support) confirms creationism?"

How, pray tell, did you draw that bit of absurdity? Or is that your feeble attempt to make an illogical link?

Evolutionists are grasping at any straw to find a path to enable their impossible replacement for God and his creation.

26 posted on 01/06/2008 11:21:44 AM PST by editor-surveyor (Turning the general election into a second Democrat primary is not a winning strategy.)
[ Post Reply | Private Reply | To 21 | View Replies]

To: Maelstorm

I like this. However, it is not the real story. We’re not ready for the real story.

27 posted on 01/06/2008 11:23:28 AM PST by RightWhale (Dean Koonz is good, but my favorite authors are Dun and Bradstreet)
[ Post Reply | Private Reply | To 1 | View Replies]

To: editor-surveyor
How, pray tell, did you draw that bit of absurdity? Or is that your feeble attempt to make an illogical link?

Once evolution has the stake in it's heart, then creationism wins. Right?

28 posted on 01/06/2008 11:45:07 AM PST by Captain Pike
[ Post Reply | Private Reply | To 26 | View Replies]

To: Captain Pike

Evolution was born dead, and the stake in its heart is science. Creationism is simply reality, and needs no win.

29 posted on 01/06/2008 12:30:23 PM PST by editor-surveyor (Turning the general election into a second Democrat primary is not a winning strategy.)
[ Post Reply | Private Reply | To 28 | View Replies]

To: RightWhale
"We’re not ready for the real story."

Its coming, ready or not...

30 posted on 01/06/2008 12:31:48 PM PST by editor-surveyor (Turning the general election into a second Democrat primary is not a winning strategy.)
[ Post Reply | Private Reply | To 27 | View Replies]

To: elephantlips

Well, we do have basketball players.

31 posted on 01/06/2008 1:20:55 PM PST by Old Professer (The critic writes with rapier pen, dips it twice, and writes again.)
[ Post Reply | Private Reply | To 5 | View Replies]

To: Fred Nerks

Problematic Chemical Postulates of the RNA World Scenario
Source: Center for the Renewal of Science and Culture
Author: Dean Kenyan
Posted on 03/07/2001 14:17:46 PST by JMJ333

‘Junk’ DNA reveals vital role [ultra-conserved sequences]
Nature Magazine | 07 May 2004 | Helen Pearson
Posted on 05/10/2004 7:01:16 AM EDT by PatrickHenry

Junk DNA may not be so junky after all
EurekAlert | March 23, 2006 | Johns Hopkins Staff
Posted on 03/29/2006 8:46:20 PM EST by DaveLoneRanger

Human genome further unravelled (’Junk’ DNA not so junky after all).
BBC | Thursday, June 14, 2007
Posted on 06/15/2007 1:49:42 PM EDT by Jedi Master Pikachu

Jumping ‘Junk’ DNA May Fuel Mammalian Evolution
(’Junk’ DNA not junk at all...ID Vindicated Again)
Scientific American | April 23, 2007 | JR Minkel
Posted on 06/21/2007 8:55:18 PM EDT by GodGunsGuts

Genetics “Central Dogma” Is Dead
Creation-Evolution Headlines | September 12, 2007
Posted on 09/16/2007 6:45:54 PM EDT by GodGunsGuts

32 posted on 01/06/2008 8:42:27 PM PST by SunkenCiv ( updated Sunday, December 30, 2007)
[ Post Reply | Private Reply | To 23 | View Replies]

To: Maelstorm


33 posted on 01/06/2008 8:51:16 PM PST by Psycho_Bunny
[ Post Reply | Private Reply | To 1 | View Replies]

To: Lucius Cornelius Sulla
My high school biology textbook mocked Lamarck; the line basically was how could those people have been so dumb as to believe in that.

After I read The Structure of Scientific Revolutions, I learned it's very dangerous to assume theories currently accepted as correct constitute a full revelation and will never change.

34 posted on 01/07/2008 9:00:47 AM PST by colorado tanker
[ Post Reply | Private Reply | To 14 | View Replies]

Navigation: use the links below to view more comments.
first previous 1-2021-34 last

Disclaimer: Opinions posted on Free Republic are those of the individual posters and do not necessarily represent the opinion of Free Republic or its management. All materials posted herein are protected by copyright law and the exemption for fair use of copyrighted works.

Free Republic
Browse · Search
Topics · Post Article

FreeRepublic, LLC, PO BOX 9771, FRESNO, CA 93794 is powered by software copyright 2000-2008 John Robinson