Posted on 12/08/2005 4:21:38 PM PST by neverdem
French biologists have just mapped out the genetic sequence of the largest known virus, and the complexity of the thing has them questioning what it is.
The genetic code of the mimivirus, as it is called, is three times longer than that of other viruses and contains elements that blur the lines between life and non-life.
Whether viruses are alive has been a matter of debate for more than half a century. They are often thought of as merely complex "biomolecules" – lifeless capsules of genetic information that must invade a living cell and hijack its machinery to reproduce.
The typical virus is 200 nanometers, or 8 millionths of an inch wide and writes its genetic code in either of two molecules: DNA or RNA, but not both.
Mimivirus, however, is more than 400 nanometers wide and has both DNA and RNA. It is so large and complex that researchers had trouble recognizing it as a virus.
"We considered it a bacteria for a year and a half," said Didier Raoult of the Universite de la Mediterranee in Marseilles, France.
Mimivirus was found during a search in 1992 for the source of an outbreak of pneumonia in Bradford, England. Although the virus was spotted inside an amoeba, it was not immediately identified and, therefore, sat in limbo for several years.
Raoult and his colleagues later obtained the mysterious "bug" and identified it as a virus a year ago. They named it mimivirus for the fact that it "mimics" bacteria. Mimivirus is, however, like other viruses in that it is not able to convert energy or replicate on its own.
The same group that identified the virus has now unraveled its genome. The work was described recently in the online version of the journal Science. Mimivirus was found to have 1.2 million base pairs – the ones and zeros of the genetic code. In this long data sequence, there are more than 1,200 genes, or "mini-programs" that give instructions for making proteins. Many bacteria do not have that many genes.
The biologists also discovered that mimivirus has seven genes common to all three of the generally-accepted domains of life: bacteria, archaea (like bacteria but slightly more complicated), and eukaryotes (everything else, from fungi to plants to animals).
There are 63 of these universal life genes. Prior to this, only one of these genes had been found in a virus.
Some of these common genes are involved in translation, which is the actual nuts and bolts of making a protein from the genetic blueprints.
"Viruses are parasites, so they can rely on the protein engineering of the host cell," Raoult said. He therefore found it "bizarre" that mimivirus would bother carrying around translation genes. The researchers are not yet sure, though, if the virus actually uses them.
Mimivirus’ unique nature may hold some key to the evolution of singe-celled organisms three billion years ago. Although it will likely take time to generate any consensus for it, the researchers advocate a fourth branch of life for mimivirus, and any other giant viruses that may turn up.
"It really is an organism," Raoult said. "You cannot believe it is just a biomolecule."
The complex interior of a Mimivirus. Electron microscopy at magnification of about 200. Credit: D. Raoult, N. Aldrovandi
A Mimivirus particle infecting an amoeba cell. Electron microscopy at magnification of about 200. Credit: D. Raoult, N. Aldrovandi
McAfee can handle this just fine.
Same thoughts ran through my head too... yay techies.
So James Carville HAS reproduced?
Here's an abstract from another analysis of the virus. It is very interesting.
Virol. 2005 Nov;79(22):14095-101.
Gene and genome duplication in Acanthamoeba polyphaga Mimivirus.
Suhre K.
Information Genomique et Structurale, UPR CNRS 2589, 31 Chemin Joseph-Aiguier, 13402 Marseille Cedex 20, France. karsten.suhre@igs.cnrs-mrs.fr
Gene duplication is key to molecular evolution in all three domains of life and may be the first step in the emergence of new gene function. It is a well-recognized feature in large DNA viruses but has not been studied extensively in the largest known virus to date, the recently discovered Acanthamoeba polyphaga Mimivirus. Here, I present a systematic analysis of gene and genome duplication events in the mimivirus genome. I found that one-third of the mimivirus genes are related to at least one other gene in the mimivirus genome, either through a large segmental genome duplication event that occurred in the more remote past or through more recent gene duplication events, which often occur in tandem. This shows that gene and genome duplication played a major role in shaping the mimivirus genome. Using multiple alignments, together with remote-homology detection methods based on Hidden Markov Model comparison, I assign putative functions to some of the paralogous gene families. I suggest that a large part of the duplicated mimivirus gene families are likely to interfere with important host cell processes, such as transcription control, protein degradation, and cell regulatory processes. My findings support the view that large DNA viruses are complex evolving organisms, possibly deeply rooted within the tree of life, and oppose the paradigm that viral evolution is dominated by lateral gene acquisition, at least in regard to large DNA viruses.
This virus is known to burn the eyes and cause severe "turtling".
Would that be the screaming mimivirus? I remember those screaming mimis man they could tear you to shreds!
Very cool. A giant intracellular parasite that has both amino acids in it. I smell a science fiction movie...
Holy cow...you could drive a fleet of nano-semi-tractor-trailor-trucks down that thing side-by-side.
No movie.
Just pay attention to the Democratic party.
Now we know what their problem is.
LVM
have we done this one?
No, we haven't. Think the list should be deployed?
Maybe our froggy friends can make a new strain of cheese with it....
I think it's pretty cool. I don't know enough to have any intelligent comments, but I'd like to hear what others say.
How insensitive of you to suggest that Democrats are parasites!
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Evolution in action?
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